No, it is not essential to use the same restriction enzymes:
(1) Some enzymes can create an identical overhang sequence, but recognize different specific sequence. Once they are ligated, you won't be able to cut the new sequence apart with both old enzyme used previously.
(2) Isoschizomers: restriction enzymes that recognize the same sequence.
(3) Blunt-end enzymes, for the same reasons above.
Restriction enzymes are site-specific, meaning they cut the DNA at very specific sites along the DNA. They read it and once they find the right place, they cleave it, leaving the "sticky ends". The sticky ends will be present in the cut sequence and where the new sequence will be integrated in the recipient. Otherwise there will be no integration.
A genetically modified organism (GMO) or genetically engineered organism (GEO) is an organism whose genetic material has been altered using genetic engineering techniques. These techniques are generally known as recombinant DNA technology. With recombinant DNA technology, DNA molecules from different sources are combined in vitro into one molecule to create a new gene. This DNA is then transferred into an organism and causes the expression of modified or novel traits.
so the cleavage sites match when you put the pieces together. If you cut at different sites, the ends won't match. You could use another enzyme if it cuts the same sequence.
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No, it is not essential to use the same restriction enzymes:
(1) Some enzymes can create an identical overhang sequence, but recognize different specific sequence. Once they are ligated, you won't be able to cut the new sequence apart with both old enzyme used previously.
(2) Isoschizomers: restriction enzymes that recognize the same sequence.
(3) Blunt-end enzymes, for the same reasons above.
Restriction enzymes are site-specific, meaning they cut the DNA at very specific sites along the DNA. They read it and once they find the right place, they cleave it, leaving the "sticky ends". The sticky ends will be present in the cut sequence and where the new sequence will be integrated in the recipient. Otherwise there will be no integration.
A genetically modified organism (GMO) or genetically engineered organism (GEO) is an organism whose genetic material has been altered using genetic engineering techniques. These techniques are generally known as recombinant DNA technology. With recombinant DNA technology, DNA molecules from different sources are combined in vitro into one molecule to create a new gene. This DNA is then transferred into an organism and causes the expression of modified or novel traits.
so the cleavage sites match when you put the pieces together. If you cut at different sites, the ends won't match. You could use another enzyme if it cuts the same sequence.